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Related Concept Videos

Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...

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Related Experiment Video

Updated: May 31, 2026

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
09:38

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

Fourier transform mass spectrometry.

Michaela Scigelova1, Martin Hornshaw, Anastassios Giannakopulos

  • 1Thermo Fisher Scientific, Bremen, Germany. Michaela.scigelova@thermofisher.com

Molecular & Cellular Proteomics : MCP
|July 12, 2011
PubMed
Summary
This summary is machine-generated.

This article introduces Fourier transform mass spectrometry (FTMS) and its applications in proteomics. It details Fourier transform ion cyclotron resonance and Orbitrap analyzers, comparing their performance for selecting the best technology.

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Last Updated: May 31, 2026

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
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Area of Science:

  • Analytical Chemistry
  • Spectrometry

Background:

  • Fourier transform mass spectrometry (FTMS) offers high mass accuracy and resolution.
  • These characteristics are crucial for interpreting complex proteomic data.

Purpose of the Study:

  • To introduce FTMS principles and applications in proteomics.
  • To describe the operation of Fourier transform ion cyclotron resonance and Orbitrap mass analyzers.
  • To compare the benefits and limitations of these FTMS technologies for practical sample analysis.

Main Methods:

  • Detailed explanation of the theory and principles of Fourier transform ion cyclotron resonance.
  • Detailed explanation of the theory and principles of Orbitrap mass analyzers.
  • Comparative analysis of performance characteristics, including mass accuracy and resolution.

Main Results:

  • Discussion of major benefits and limitations of FTMS technology in proteomic applications.
  • Illustrations and examples provided to demonstrate practical sample analysis.
  • Performance differences between Fourier transform ion cyclotron resonance and Orbitrap highlighted.

Conclusions:

  • Guidance on selecting the most appropriate FTMS technology based on application needs.
  • Overview of recent developments and future outlook for high-performing mass spectrometers.