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Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
Published on: January 8, 2015
Excision of the En/Spm transposable element of Zea mays requires two element-encoded proteins
1Max-Planck-Institut für Züchtungsforschung, Molekulare Pflanzengenetik, Köln, FRG.
Abstract:
An excision assay system for En/Spm was developed in transgenic tobacco. The characteristics of excision and integration are similar to the natural system of Zea mays. In this transgenic model system two En/Spm encoded trans-acting functions, TNPA and TNPD, are required for excision. A biochemical model for transposition is proposed that might also be applicable to other transposable elements.
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