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Published on: March 18, 2012
Highly sensitive spectrofluorimetic determination of rutin based on its activated effect on a multi-enzyme redox
1Department of Chemistry, Zhoukou Normal University, Zhoukou, 466000, People's Republic of China. chen-yh75@163.com
Abstract:
A highly sensitive and simple spectrofluorimetric method for the determination of rutin, based on its activated effect on a haemoglobin-catalysed reaction, was developed. Under optimum conditions, the concentration of rutin was linear, with decreased fluorescence (ΔF) of the system under optimal experimental conditions. The calibration graph was linear in the range 1.0 × 10(-7) -3.0 × 10(-5) mol/L, with a detection limit of 7.0 × 10(-8) mol/L. The relative standard deviation (RSD) was 3.26% for 11 determinations of 1.0 × 10(-5) mol/L. This method was used for the determination of rutin in pharmaceuticals with satisfactory results.
