Related Experiment Video
Updated: Aug 8, 2026

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells
Published on: July 22, 2013
[Regulation by biogenic amines of energy functions of mitochondria]
Abstract:
Biogenic amines (phenylethylamine, tyramine, dopamine, tryptamine, serotonin and spermine) decreased activities of the rotenone-insensitive NADH-cytochrome c reductase, the succinate cytochrome c reductase and the succinate dehydrogenase in incubation mixtures containing mitochondrial membranes and the monoamine oxidase inhibitors chlorgyline and deprenyl. Spermine exhibited its influences at lower concentrations as compared with other monoamines. The effect of monoamines depended on presence of hydroxyl group (or groups) in the aromatic ring which led to elevation of the inhibitory effects. Lysis of membranes by Triton X-100 prevented the inhibitory action of all the compounds studied on succinate dehydrogenase. Alterations in content of some biogenic amines under pathological conditions and especially in drug inhibition of the monoamine oxidases appear to influence the energy functions of mitochondria, possibly via changes in the surface charge of the membranes.
Related Concept Videos
Mitochondria
Electron Transport Chain: Complex I and II
ROS generation is regulated and maintained at moderate levels necessary...
Translocation of Proteins into the Mitochondria
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
Regulation of Metabolism
Mitochondria
Introduction to Metabolism

