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Updated: May 31, 2026

In vitro Cell Migration and Invasion Assays
09:55

In vitro Cell Migration and Invasion Assays

Published on: June 1, 2014

Transwell(®) invasion assays.

John Marshall1

  • 1Barts Cancer Institute, Barts and The London, Queen Mary's School of Medicine and Dentistry, Queen Mary, University of London, London, UK. j.f.marshall@qmul.ac.uk

Methods in Molecular Biology (Clifton, N.J.)
|July 13, 2011
PubMed
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Quantitative in vitro invasion assays, like Transwell(®) Permeable Supports, are crucial for identifying cancer invasion inhibitors. These assays use a cell-permeable membrane to measure tumor cell invasion towards a chemoattractant.

Area of Science:

  • Oncology
  • Cell Biology
  • Biotechnology

Background:

  • Identifying inhibitors of cancer invasion is critical.
  • Quantitative in vitro invasion assays are essential tools for this research.
  • The Boyden assay system and its modern adaptations, such as Transwell(®) Permeable Supports, are widely used.

Purpose of the Study:

  • To provide protocols for performing Transwell invasion assays.
  • To discuss the limitations of current invasion assay designs.
  • To highlight the need for improved matrices and the inclusion of tumor microenvironment cells.

Main Methods:

  • Utilizing Transwell(®) Permeable Supports, a two-chamber system separated by a cell-permeable membrane.
  • Blocking the membrane pores with an extracellular matrix gel to simulate in vivo conditions.

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Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol

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Last Updated: May 31, 2026

In vitro Cell Migration and Invasion Assays
09:55

In vitro Cell Migration and Invasion Assays

Published on: June 1, 2014

Modeling and Imaging 3-Dimensional Collective Cell Invasion
07:08

Modeling and Imaging 3-Dimensional Collective Cell Invasion

Published on: December 7, 2011

Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol
06:14

Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol

Published on: March 12, 2018

  • Quantifying tumor cell invasion by counting cells that traverse the membrane towards a chemoattractant.

    • Main Results:

    • Transwell(®) assays provide accurate and repeatable measurements of cancer cell invasion.
    • The current assay designs have limitations regarding the available matrices.
    • Existing assays lack the inclusion of crucial tumor microenvironment cells.

    Conclusions:

    • Transwell(®) invasion assays are valuable for cancer research but require further refinement.
    • Improvements in matrix composition and the incorporation of tumor microenvironment components are needed.
    • Enhanced assay designs will lead to more accurate identification of cancer invasion inhibitors.