Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nectin receptors mediate mechanosensing to YAP via AKT.

Communications biology·2026
Same author

CoCB cell signalling 2024.

Current opinion in cell biology·2026
Same author

A rapid method of evaluating cytotoxic drug efficacy using sub-cellular fluctuation imaging.

Scientific reports·2025
Same author

Phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K) inhibitors reduce vascular inflammation in vitro and in vivo.

British journal of pharmacology·2025
Same author

Sos1 ablation alters focal adhesion dynamics and increases Mmp2/9-dependent gelatinase activity in primary mouse embryonic fibroblasts.

Cell communication and signaling : CCS·2025
Same author

Pharmacological inhibition of ezrin reduces proliferative and invasive phenotype in acute lymphoblastic leukemia cells.

European journal of pharmacology·2024
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: May 31, 2026

Analysis of Cell Migration within a Three-dimensional Collagen Matrix
08:02

Analysis of Cell Migration within a Three-dimensional Collagen Matrix

Published on: October 5, 2014

Quantification of transendothelial migration using three-dimensional confocal microscopy.

Robert J Cain1, Bárbara Borda d'Água, Anne J Ridley

  • 1Cell Motility and Cytoskeleton Group, Randall Division of Cell and Molecular Biophysics, King’s College London, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|July 13, 2011
PubMed
Summary
This summary is machine-generated.

This study presents a confocal microscopy method to visualize cell migration across endothelial barriers. The technique quantifies transendothelial migration (TEM) and subendothelial matrix invasion for leukocytes and cancer cells.

More Related Videos

Quantifying the Brain Metastatic Tumor Micro-Environment using an Organ-On-A Chip 3D Model, Machine Learning, and Confocal Tomography
09:53

Quantifying the Brain Metastatic Tumor Micro-Environment using an Organ-On-A Chip 3D Model, Machine Learning, and Confocal Tomography

Published on: August 16, 2020

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
09:16

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Published on: November 26, 2018

Related Experiment Videos

Last Updated: May 31, 2026

Analysis of Cell Migration within a Three-dimensional Collagen Matrix
08:02

Analysis of Cell Migration within a Three-dimensional Collagen Matrix

Published on: October 5, 2014

Quantifying the Brain Metastatic Tumor Micro-Environment using an Organ-On-A Chip 3D Model, Machine Learning, and Confocal Tomography
09:53

Quantifying the Brain Metastatic Tumor Micro-Environment using an Organ-On-A Chip 3D Model, Machine Learning, and Confocal Tomography

Published on: August 16, 2020

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
09:16

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Published on: November 26, 2018

Area of Science:

  • Cell Biology
  • Biophysics
  • Pathology

Background:

  • Transendothelial migration (TEM) is crucial in inflammation and cancer metastasis.
  • Existing in vitro models lack detailed morphology and quantitative TEM data.
  • Advanced imaging is vital for studying cell movement in physiological processes.

Purpose of the Study:

  • To describe a confocal microscopy method for assessing TEM.
  • To provide detailed visual and quantitative data on cell migration.
  • To model cell migration across endothelial barriers and into subendothelial matrix.

Main Methods:

  • Confocal microscopy-based assay.
  • Assessment of leukocyte and cancer cell TEM across endothelial barriers.
  • Analysis of migration into a subendothelial extracellular matrix.

Main Results:

  • The method provides detailed visual information on cell morphologies.
  • Quantitative data on the rate of TEM is obtainable.
  • The assay allows assessment of migration into a biomimetic subendothelial matrix.

Conclusions:

  • Confocal microscopy offers a powerful tool for studying TEM.
  • This method enhances understanding of cell migration in disease.
  • The technique bridges in vitro modeling with in vivo relevance.