Related Experiment Video
Updated: May 31, 2026

06:57
RNAi Mediated Gene Knockdown and Transgenesis by Microinjection in the Necromenic Nematode Pristionchus pacificus
Published on: October 16, 2011
RNA silencing in Monterey.
Olivia S Rissland1, Eric C Lai
1Whitehead Institute, Massachusetts Institute of Technology, Cambridge, MA 02142, USA. rissland@wi.mit.edu
Summary
The 2011 Keystone Symposium on Mechanism and Biology of Silencing showcased advancements in gene silencing. Discussions highlighted progress and future directions in this dynamic research area.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- The Keystone Symposia convene leading researchers to discuss cutting-edge scientific advancements.
- Gene silencing mechanisms are crucial for various biological processes, including development and disease.
- Understanding these mechanisms is vital for therapeutic interventions.
Purpose of the Study:
- To summarize key findings and discussions from the 2011 Keystone Symposium on the Mechanism and Biology of Silencing.
- To highlight emerging trends and future research directions in the field of gene silencing.
- To provide an overview of the scientific progress presented at the meeting.
Main Methods:
- The abstract does not detail specific experimental methods but summarizes presentations and discussions.
- It reflects the collective knowledge and findings shared among experts in the field.
- Information is synthesized from oral and poster presentations at the symposium.
Main Results:
- The symposium featured vigorous scientific discussions on the mechanism and biology of silencing.
- Key findings and emerging trends in gene silencing research were presented.
- The meeting stimulated new ideas and pushed the field into new territories.
Conclusions:
- The 2011 Keystone Symposium successfully fostered collaboration and knowledge exchange.
- Advancements in understanding gene silencing mechanisms were evident.
- The field of gene silencing is dynamic and expanding with promising future directions.
Related Concept Videos
Experimental RNAi
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
siRNA - Small Interfering RNAs
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

