Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Effective Treatment using Bumped Kinase Inhibitor 1708 in a Calf Clinical Model of Cryptosporidiosis.

The Journal of infectious diseases·2026

Same author

<i>In vitro</i> activity of MRS-2541, a novel MetRS inhibitor, against a selection of resistant gram-positive organisms associated with serious hospital infections.

Microbiology spectrum·2026

Same author

Coincident maps of changing land cover, land use, and forest condition in the United States, 1985-present.

Scientific data·2026

Same author

CSN5i-3 is an orthosteric molecular glue inhibitor of COP9 signalosome.

Nature·2026

Same author

Correction: Randomization in the age of platform trials: unexplored challenges and some potential solutions.

BMC medical research methodology·2026

Same author

Resolving Clinically Indeterminate Findings During Anal Cancer Surveillance with TTMV-HPV DNA.

Cancers·2026

Same journal

A robust and validated method for the determination of 21 urinary metabolites of 15 plasticizers, including phthalates, DEHTP, and DINCH, by online SPE and liquid chromatography-tandem mass spectrometry.

Analytical and bioanalytical chemistry·2026

Same journal

A label-free membrane-based biosensor array with AuNP-modified PDMS for sensitive and specific detection of alpha-fetoprotein.

Analytical and bioanalytical chemistry·2026

Same journal

Smartphone-integrated one-step colorimetric glucose detection at physiological pH enabled by a haloperoxidase mimic.

Analytical and bioanalytical chemistry·2026

Same journal

Chemiluminescence functionalized magnetic nanoparticles-based biosensor for sensitive detection of glucose, uric acid, and cholesterol.

Analytical and bioanalytical chemistry·2026

Same journal

Single-cell mass spectrometry imaging: platform advances for multimodal spatial omics.

Analytical and bioanalytical chemistry·2026

Same journal

Advancing total uronic acid quantification using a stable isotope dilution approach: validation and application to plant- and algal-derived polysaccharides.

Analytical and bioanalytical chemistry·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 31, 2026

NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode

NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode

Published on: June 4, 2021

Screening a fragment cocktail library using ultrafiltration.

Sayaka Shibata¹, Zhongsheng Zhang, Konstantin V Korotkov

¹Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.

Analytical and Bioanalytical Chemistry

|July 14, 2011

Summary

This summary is machine-generated.

Ultrafiltration screening identifies potential drug ligands for protein targets. This fragment-based drug discovery method is efficient for targets with typical binding affinities.

More Related Videos

Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery

Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery

Published on: May 16, 2021

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin

Published on: March 3, 2021

Related Experiment Videos

Last Updated: May 31, 2026

NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode

NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode

Published on: June 4, 2021

Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery

Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery

Published on: May 16, 2021

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin

Published on: March 3, 2021

Area of Science:

Biochemistry
Molecular Biology
Drug Discovery

Background:

Fragment-based drug discovery (FBDD) is crucial for identifying novel therapeutic leads.
Identifying ligands with millimolar to micromolar dissociation constants (K(d)) is a common challenge in FBDD.
High-throughput screening methods are needed to accelerate ligand discovery for protein targets.

Purpose of the Study:

To adapt ultrafiltration for a 96-well format suitable for FBDD.
To screen fragment-sized molecules against medical structural genomics target proteins.
To validate the ultrafiltration method for identifying protein-ligand interactions.

Main Methods:

Ultrafiltration coupled with a 96-well format was employed.
Cocktails of small molecules (150-300 Da) were screened.
Competitive binding assays were used to confirm ligand interactions.

Main Results:

The ultrafiltration method successfully identified potential ligands for protein targets.
Screening was effective for identifying molecules within the typical FBDD affinity range.
Method validation confirmed the reliability of identified interactions.

Conclusions:

Ultrafiltration is a versatile and effective method for fragment-based drug discovery.
The 96-well format enhances throughput for screening small molecule libraries.
This approach aids in identifying novel ligands for protein targets in structural genomics.