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Updated: May 31, 2026

Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins

Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins

Published on: February 8, 2019

Ustilago maydis transcript features identified through full-length cDNA analysis.

Colleen E Doyle¹, Michael E Donaldson, Erin N Morrison

¹Environmental and Life Sciences Graduate Program, Trent University, Peterborough, ON K9J 7B8, Canada.

Molecular Genetics and Genomics : MGG

|July 14, 2011

Summary

This summary is machine-generated.

This study enhances the Ustilago maydis genome annotation by analyzing full-length cDNA sequences. New insights reveal gene features, UTR characteristics, and transcriptional control mechanisms in this important plant pathogen.

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A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines

A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines

Published on: January 3, 2014

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Last Updated: May 31, 2026

Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins

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Published on: February 8, 2019

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

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Published on: September 30, 2016

A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines

A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines

Published on: January 3, 2014

Area of Science:

Mycology
Plant Pathology
Genomics

Background:

Ustilago maydis is a key model organism for studying basidiomycete biotrophic plant pathogens.
Accurate genome annotation is crucial for understanding pathogen biology and developing control strategies.

Purpose of the Study:

To improve the genome annotation of Ustilago maydis.
To construct comprehensive databases for promoter and untranslated regions (UTRs).
To identify novel sequence features and regulatory elements.

Main Methods:

Utilized 12,943 full-length cDNA sequences for genome annotation.
Assembled expressed sequence tags (ESTs) and full cDNA sequences into contigs.
Sequenced a subset of clones to determine full cDNA sequences.

Main Results:

Confirmed 2,842 gene models, with 690 containing introns.
Identified over 500 potential upstream open reading frames (ORFs) and one new potential ORF.
Correlated UTR length with transcript representation and gene function, revealing differences from Saccharomyces cerevisiae.

Conclusions:

The study provides a significantly improved Ustilago maydis gene annotation.
Discovered novel sequence features, including UTR characteristics and potential regulatory elements.
Offered new insights into transcriptional control and mRNA maturation in Ustilago maydis.