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Updated: May 31, 2026
09:41
Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
Published on: March 17, 2023
Inflammation inhibits GPR81 expression in adipose tissue
Kenneth R Feingold1, Arthur Moser, Judy K Shigenaga
1Metabolism Section (111F), Department of Veterans Affairs Medical Center, University of California San Francisco, 4150 Clement Street, San Francisco, CA 94121, USA. kenneth.feingold@ucsf.edu
Summary
Inflammation significantly reduces G protein-coupled receptor 81 (GPR81) expression in mouse adipose tissue. This finding is relevant for understanding metabolic disorders and inflammation.
Area of Science:
- Metabolic research
- Inflammation studies
- Adipose tissue biology
Background:
- G protein-coupled receptor 81 (GPR81) is activated by lactate, inhibiting lipolysis.
- Understanding GPR81 regulation in adipose tissue is crucial for metabolic health.
Purpose of the Study:
- To investigate the impact of inflammatory stimuli on GPR81 expression in mouse adipose tissue.
- To explore the relationship between inflammation and GPR81 levels in metabolic conditions.
Main Methods:
- Mice were treated with inflammatory agents like lipopolysaccharide (LPS), zymosan, or turpentine.
- GPR81 mRNA levels were quantified using real-time PCR.
- In vitro studies involved treating 3T3-L1 adipocytes with cytokines.
Main Results:
- LPS, zymosan, and turpentine treatments decreased GPR81 mRNA levels in mouse adipose tissue.
- No significant change in GPR81 expression was observed in adipocytes treated with cytokines.
- GPR81 expression was reduced in ob/ob mice, a model for type 2 diabetes with inflammation.
Conclusions:
- Inflammatory conditions lead to a decrease in GPR81 expression within adipose tissue.
- These findings suggest a link between inflammation, GPR81 regulation, and metabolic dysfunction.
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