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Updated: May 31, 2026
13:15
Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
Published on: June 1, 2011
A lateral flow biosensor for rapid detection of DNA-binding protein c-jun
Zhiyuan Fang1, Chenchen Ge, Wenjuan Zhang
1South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.
Biosensors & Bioelectronics
|July 15, 2011
Summary
A novel lateral flow biosensor rapidly detects DNA-binding proteins like c-jun using gold nanoparticles and antibodies. This immunochromatographic assay provides visual detection within 10 minutes for biological research.
Area of Science:
- Biotechnology
- Biosensor Technology
- Molecular Biology
Background:
- Detection of DNA-binding proteins is crucial for understanding gene regulation.
- Existing methods can be time-consuming and require specialized equipment.
- Rapid and specific detection of these proteins is needed for various research applications.
Purpose of the Study:
- To develop a rapid, visual, and specific lateral flow biosensor for DNA-binding proteins.
- To utilize gold nanoparticles and immunochromatographic principles for detection.
- To validate the biosensor's performance using a specific target protein, c-jun.
Main Methods:
- Developed a lateral flow biosensor incorporating a sample pad, conjugate pad, nitrocellulose membrane, and absorbent pad.
- Coated gold nanoparticles with a DNA probe containing a protein binding consensus sequence.
- Immobilized an antibody against the target DNA-binding protein onto the nitrocellulose membrane's test zone.
Main Results:
- The biosensor successfully detected the DNA-binding protein c-jun.
- Achieved rapid detection (within 10 minutes) down to 0.2 footprint units of c-jun.
- Visually detected c-jun activity in HeLa cell lysates with high specificity, distinguishing it from other proteins.
Conclusions:
- The developed lateral flow biosensor offers a fast and visually detectable method for identifying DNA-binding proteins.
- This technology has potential applications in molecular diagnostics and biological research.
- The biosensor demonstrates high specificity and sensitivity for target protein detection.
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