Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ampicillin serves as an electron donor.

S Umeki1

  • 1Department of Medicine, Kawasaki Medical School, Okayama, Japan.

The International Journal of Biochemistry
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inferior oblique muscle of the eye: its foetal development with special reference to understanding of the frequent variants in adults.

Folia morphologica·2021
Same author

Investigation of the cytotoxic effect of flavopiridol in canine lymphoma cell lines.

Veterinary and comparative oncology·2015
Same author

The effects of oncolytic reovirus in canine lymphoma cell lines.

Veterinary and comparative oncology·2014
Same author

Characterization of mutant clones lacking T-cell receptor alleles in a cell line from an adult T-cell leukaemia patient.

British journal of haematology·2001
Same author

Expression of heparin-binding epidermal growth factor-like growth factor in the hepatocytes of fibrotic rat liver during hepatocarcinogenesis.

Journal of gastroenterology and hepatology·2000
Same author

Effects of exogenous human heparin-binding epidermal growth factor-like growth factor on DNA synthesis of hepatocytes in normal mouse liver.

Biochemical and biophysical research communications·1999
Same journal

Intracellular localization of dinucleosideoligophosphate phosphorylase from Euglena gracilis.

The International journal of biochemistry·2010
Same journal

Specific phosphorylase from Euglena gracilis splits diadenosine 5',5'''-P(1),P(4)-tetraphosphate (Ap(4)A) and diadenosine 5',5'''-P(1),P(3)-triphosphate (Ap(3)A).

The International journal of biochemistry·2010
Same journal

Immunochemical homologies among L-alpha-hydroxyacid oxidase isozymes.

The International journal of biochemistry·2010
Same journal

Biochemical characterization of hemorrhagic toxin from Crotalus viridis viridis (prairie rattlesnake) venom.

The International journal of biochemistry·1994
Same journal

The 5'-sequence of the murine Hox-b3 (Hox-2.7) gene and its intron contain multiple transcription-regulatory elements.

The International journal of biochemistry·1994
Same journal

Characterization of human arylsulfatase A glycans.

The International journal of biochemistry·1994
See all related articles

Ampicillin did not affect neutrophil superoxide production but increased cytochrome c reduction. These findings suggest ampicillin may act as an electron donor or superoxide generator in biological systems.

Area of Science:

  • Immunology
  • Biochemistry

Background:

  • Neutrophils play a crucial role in the innate immune response.
  • Superoxide production by neutrophils is a key component of their antimicrobial activity.
  • Cytochrome c reduction is an indicator of electron transport chain activity.

Purpose of the Study:

  • To investigate the impact of ampicillin on human neutrophil function.
  • To determine if ampicillin influences superoxide production or cytochrome c reduction.

Main Methods:

  • Human neutrophils were isolated and stimulated with phorbol myristate acetate (PMA).
  • The effects of ampicillin on superoxide production were measured.
  • Cytochrome c reduction was assessed in the presence of varying ampicillin concentrations.

Related Experiment Videos

Main Results:

  • Ampicillin did not stimulate or amplify PMA-induced superoxide production in neutrophils.
  • Ampicillin demonstrated a dose-dependent increase in cytochrome c reduction.
  • Specific measurements indicated both superoxide dismutase-inhibitable and non-inhibitable cytochrome c reduction.

Conclusions:

  • Ampicillin does not directly enhance neutrophil oxidative burst.
  • Ampicillin appears to interact with cellular electron transport pathways.
  • The results suggest ampicillin may function as an electron donor or superoxide generator.