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The effects of oncolytic reovirus in canine lymphoma cell lines

C C Hwang1, S Umeki1, M Igase2

  • 1Laboratory of Molecular Diagnostics and Therapeutics, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.

Veterinary and Comparative Oncology
|October 17, 2014
PubMed

Insights

Reovirus shows potential as an oncolytic virus for canine lymphoma, though less susceptible than canine mast cell tumors. Further research is needed to optimize its use in veterinary oncology.

Area of Science:

  • Veterinary Oncology
  • Virology
  • Molecular Biology

Background:

  • Reovirus demonstrates oncolytic potential in human cancers and canine mast cell tumors.
  • Exploring novel viral therapies for canine cancers is crucial.

Purpose of the Study:

  • To evaluate the susceptibility of canine lymphoma cell lines to reovirus.
  • To investigate reovirus replication, infectivity, and cell death mechanisms in canine lymphoma.

Main Methods:

  • Testing reovirus on 10 canine lymphoma cell lines.
  • Assessing virus replication, infectivity, and apoptosis (propidium iodide staining, Annexin V-FITC/PI assay, PARP cleavage).
  • Evaluating tumor suppression in NOD/SCID mice after intratumoral reovirus injection.

Main Results:

  • Reovirus induced cell death, replication, and infectivity in four of ten canine lymphoma cell lines.
  • Apoptosis was confirmed as the mechanism of cell death in susceptible cell lines.
  • Reovirus injection suppressed subcutaneous canine lymphoma tumor growth in mice.
  • Canine lymphoma exhibited lower susceptibility to reovirus compared to canine mast cell tumors.

Conclusions:

  • Reovirus exhibits oncolytic activity against a subset of canine lymphoma cell lines.
  • Canine lymphoma is less susceptible to reovirus than canine mast cell tumors.
  • Reovirus warrants further investigation for veterinary oncology applications in canine lymphoma.
Keywords:
Rasapoptosiscanine lymphomaoncolytic virotherapyreovirus

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