Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview of Cell Signaling

Overview of Cell Signaling

Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate with the environment.
Cells respond to many types of information, often through receptor proteins positioned on the membrane. For example, skin cells respond to and transmit touch...

Overview of Cell Signaling

Overview of Cell Signaling

Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate with the environment.
Cells respond to many types of information, often through receptor proteins positioned on the membrane. For example, skin cells respond to and transmit touch...

What is the Cell Cycle?

What is the Cell Cycle?

The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the original...

What is the Cell Cycle?

What is the Cell Cycle?

The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: the interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the original...

What is the Cell Cycle?

What is the Cell Cycle?

The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: the interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the original...

What is the Cell Cycle?

What is the Cell Cycle?

The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the original...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The Origin of RNA and the Formose-Ribose-RNA Pathway.

International journal of molecular sciences·2024

Same author

Apoptotic Janus-faced mycotoxins against thoracal and breast metastases.

Apoptosis : an international journal on programmed cell death·2023

Same author

Macromolecular Structure of Linearly Arranged Eukaryotic Chromosomes.

International journal of molecular sciences·2022

Same author

Janus-Faced Molecules against Plant Pathogenic Fungi.

International journal of molecular sciences·2021

Same author

Prebiotic Pathway from Ribose to RNA Formation.

International journal of molecular sciences·2021

Same author

MTT Test and Time-lapse Microscopy to Evaluate the Antitumor Potential of Nucleoside Analogues.

Anticancer research·2021

Search research articles

Related Experiment Video

Updated: May 31, 2026

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Overview of cell synchronization.

Gaspar Banfalvi¹

¹Department of Microbial Biotechnology and Cell Biology, University of Debrecen, 4010, Debrecen, Hungary. bgaspar@delfin.klte.hu

Methods in Molecular Biology (Clifton, N.J.)

|July 15, 2011

Summary

Cell synchronization techniques are crucial for studying cell cycle regulation and developing new drugs. This review compiles and updates methods for cell cycle control, aiding researchers in selecting optimal synchronization strategies.

Area of Science:

Cell Biology
Molecular Biology

Background:

Cell cycle regulation is key to understanding cellular processes.
Synchronized cell populations are vital for studying macromolecular biosynthesis, protein phosphorylation, and drug development.

Purpose of the Study:

To provide a comprehensive and updated compilation of cell synchronization techniques.
To offer insights into the basic concepts, criteria, and applications of cell synchronization.

Main Methods:

Physical fractionation methods including flow cytometry, dielectrophoresis, and cytofluorometric purification.
Chemical blockade methods for cell cycle arrest.
Low-temperature synchronization techniques.
Synchronization of unicellular organisms.

More Related Videos

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Alignment of Synchronized Time-Series Data Using the Characterizing Loss of Cell Cycle Synchrony Model for Cross-Experiment Comparisons

Alignment of Synchronized Time-Series Data Using the Characterizing Loss of Cell Cycle Synchrony Model for Cross-Experiment Comparisons

Published on: June 9, 2023

Related Experiment Videos

Last Updated: May 31, 2026

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Alignment of Synchronized Time-Series Data Using the Characterizing Loss of Cell Cycle Synchrony Model for Cross-Experiment Comparisons

Alignment of Synchronized Time-Series Data Using the Characterizing Loss of Cell Cycle Synchrony Model for Cross-Experiment Comparisons

Published on: June 9, 2023

Main Results:

Detailed overview of various cell synchronization methods.
Comparison of different cell synchrony techniques.
Discussion on the impact of synchronization on transfection efficiency.

Conclusions:

Effective cell synchronization is essential for advancing research in cell cycle regulation and related fields.
This review serves as a valuable resource for scientists seeking to understand and apply cell synchronization methods.