Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

The Cell Cycle Control System

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...

Cells Coordinate Growth and Proliferation

Cells Coordinate Growth and Proliferation

Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Liver transcriptome dynamics in Holstein cows during the periparturient transition.

Scientific reports·2026

Same author

Phased-assembly-driven pangenome graphs for structural variant genotyping and complex trait mapping in dairy cattle.

Nature communications·2026

Same author

Genome-wide identification and functional analysis of metallothionein family genes in response to cadmium/copper exposure in the unicellular eukaryote Paramecium.

Ecotoxicology and environmental safety·2026

Same author

Mapping the cerebral structural changes related to the multi-dimensional neuropsychiatric deficits in patients with ischemic thalamic stroke.

Brain research bulletin·2025

Same author

A multi-tissue single-cell expression atlas in cattle.

Nature genetics·2025

Same author

Physiological and molecular responses to different sizes of polystyrene micro/nanoplastics in the model unicellular eukaryote Paramecium tetraurelia.

Journal of hazardous materials·2025

Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 31, 2026

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

Specific cell cycle synchronization with butyrate and cell cycle analysis.

¹Bovine Functional Genomics Laboratory, Animal and Natural Resources Institute, ARS, USDA, 20705, Beltsville, MD, USA. congjun.li@ars.usda.gov

Methods in Molecular Biology (Clifton, N.J.)

|July 15, 2011

Summary

This summary is machine-generated.

Butyrate can reversibly arrest Madin Darby Bovine Kidney (MDBK) cells at the G1 phase, enabling synchronized cell cycle studies. This method allows for precise analysis of cell proliferation and DNA synthesis.

More Related Videos

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Related Experiment Videos

Last Updated: May 31, 2026

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

Synchronized cells are crucial for studying cell cycle progression and proliferation.
Butyrate is known to induce cell cycle arrest and apoptosis in various cell types.

Purpose of the Study:

To investigate the potential of butyrate as an agent for inducing cell cycle synchronization in Madin Darby Bovine Kidney (MDBK) cells.
To characterize the specific stage of the cell cycle affected by butyrate and the reversibility of the arrest.

Main Methods:

Madin Darby Bovine Kidney (MDBK) cells were treated with 10 mM butyrate.
Cell cycle arrest and growth inhibition were analyzed using 5-bromo-2'-deoxyuridine (BrdU) incorporation and flow cytometry.
Reversibility of butyrate-induced arrest was assessed after removal of the compound.

Main Results:

Butyrate treatment induced reversible growth inhibition and cell cycle arrest in MDBK cells.
The arrest occurred at a specific point immediately post-mitosis and early in the G1 phase.
Following butyrate removal, cells synchronized and initiated DNA synthesis (S phase) within 8 hours, with a total cell cycle of approximately 20 hours.

Conclusions:

Butyrate is an effective agent for achieving G1-phase synchronization in MDBK cells.
The combination of BrdU incorporation and DNA content analysis provides a robust method to eliminate population overlap and precisely determine DNA synthesis dynamics.
This synchronized cell model facilitates detailed studies of cell proliferation and cell cycle regulation.