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Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation
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Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation

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Human adipose tissue derived mesenchymal stem cells are resistant to several chemotherapeutic agents

Wei Liang1, Hailong Xia, Jing Li

  • 1Department of Oncology, Center for Cell Therapy and Tissue Engineering, The First Affiliated Hospital of Anhui Medical University, 218# Jixi Road, Hefei, 230022, Anhui Province, People's Republic of China, williamleung@163.com.

Cytotechnology
|July 16, 2011
PubMed

Insights

Human adipose-derived mesenchymal stem cells (ADMSCs) show resistance to common chemotherapy drugs like cisplatin, camptothecin, and vincristine. These stem cells fully recover and maintain their characteristics after drug exposure.

Area of Science:

  • Stem cell biology
  • Cancer research
  • Pharmacology

Background:

  • Human adipose-derived mesenchymal stem cells (ADMSCs) are multipotent stem cells with therapeutic potential.
  • The response of ADMSCs to chemotherapeutic agents is not well understood.
  • Understanding ADMSC response to chemotherapy is crucial for cell-based therapies in cancer patients.

Purpose of the Study:

  • To assess the acute effects of common chemotherapeutic agents on ADMSCs.
  • To compare the response of ADMSCs to a testicular germ cell tumor (TGCT) cell line.
  • To evaluate the recovery and stem cell characteristic maintenance of ADMSCs post-chemotherapy.

Main Methods:

  • In vitro culture of human ADMSCs and a TGCT cell line.
  • Exposure of cells to cisplatin, camptothecin, and vincristine.
  • Assessment of cell number recovery and stem cell marker expression.

Main Results:

  • Human ADMSCs demonstrated resistance to cisplatin, camptothecin, and vincristine.
  • ADMSCs exhibited full recovery of cell numbers after exposure to these agents.
  • Stem cell characteristics of ADMSCs were preserved in vitro following chemotherapy exposure.

Conclusions:

  • ADMSCs are resistant to commonly used chemotherapeutic agents.
  • ADMSCs possess robust recovery capabilities and maintain stemness after chemotherapy.
  • These findings support the potential use of ADMSCs in cell therapies, even in patients undergoing chemotherapy.

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