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Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...

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Related Experiment Video

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Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation (BiFC-PALM)
12:42

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation (BiFC-PALM)

Published on: December 22, 2015

Gene expression using the PALM system.

Jian-Xin Lu1, Cheuk-Chun Szeto

  • 1Department of Medicine & Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong, China.

Methods in Molecular Biology (Clifton, N.J.)
|July 16, 2011
PubMed
Summary
This summary is machine-generated.

The PALM Robot MicroBeam system precisely isolates cells from tissues for RNA analysis. This method aids in understanding gene expression in specific cell types for various biological studies.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genomics

Background:

  • Laser microdissection is crucial for isolating specific cells from complex biological samples.
  • Understanding cell-specific gene expression is key to elucidating physiological and pathological processes.

Purpose of the Study:

  • To detail the application of the PALM Robot MicroBeam system for isolating RNA from specific cells within complex tissues.
  • To provide protocols for gene expression analysis using laser microdissected cells.

Main Methods:

  • Utilizing the PALM Robot MicroBeam laser microdissection system for rapid and precise cell isolation.
  • Implementing optimized protocols for tissue preparation and cell microdissection.
  • Performing subsequent RNA isolation and gene expression analysis.

Main Results:

  • Successful isolation of RNA from limited cell populations (500-10,000 cells) using the PALM MicroBeam system.
  • Demonstration of a workflow from tissue preparation to quantitative gene expression results.
  • Emphasis on critical steps for obtaining reliable data from microdissected cells.

Conclusions:

  • The PALM MicroBeam system is an effective tool for isolating specific cells for gene expression studies.
  • Optimized protocols are essential for reliable gene expression analysis from limited cell samples.
  • This technique facilitates the study of specialized cell types in health and disease.