Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Treatment monitoring by biomarker analysis in a Phase I dose-expansion study of AZD2811 for relapsed/refractory small-cell lung cancer.

British journal of cancer·2026
Same author

Society for Immunotherapy of Cancer: Standards for Reporting of Multiplex Immunohistochemistry/Immunofluorescence Assays (STORMI).

Journal for immunotherapy of cancer·2025
Same author

Multi-omic profiling provides insights into the heterogeneity, microenvironmental features, and biomarker landscape of small-cell lung cancer.

Molecular cancer·2025
Same author

A 3D Self-Assembly Platform Integrating Decellularized Matrix Recapitulates In Vivo Tumor Phenotypes and Heterogeneity.

Cancer research·2025
Same author

Engineering Tumor Stroma Morphogenesis Using Dynamic Cell-Matrix Spheroid Assembly.

bioRxiv : the preprint server for biology·2024
Same author

Molecular classification and biomarkers of outcome with immunotherapy in extensive-stage small-cell lung cancer: analyses of the CASPIAN phase 3 study.

Molecular cancer·2024
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: May 31, 2026

Automated Dissection Protocol for Tumor Enrichment in Low Tumor Content Tissues
06:44

Automated Dissection Protocol for Tumor Enrichment in Low Tumor Content Tissues

Published on: March 29, 2021

Immunoguided microdissection techniques.

Michael A Tangrea1, Jeffrey C Hanson, Robert F Bonner

  • 1Pathogenetics Unit and Laser Microdissection Core, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)
|July 16, 2011
PubMed
Summary
This summary is machine-generated.

Immunohistochemistry and immunofluorescence guide laser microdissection for precise cell isolation. This automated approach is essential for large-scale studies and rare cell identification in heterogeneous tissues.

More Related Videos

A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas
07:42

A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas

Published on: June 5, 2014

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment
13:01

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment

Published on: June 3, 2022

Related Experiment Videos

Last Updated: May 31, 2026

Automated Dissection Protocol for Tumor Enrichment in Low Tumor Content Tissues
06:44

Automated Dissection Protocol for Tumor Enrichment in Low Tumor Content Tissues

Published on: March 29, 2021

A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas
07:42

A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas

Published on: June 5, 2014

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment
13:01

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment

Published on: June 3, 2022

Area of Science:

  • Biotechnology
  • Cell Biology
  • Histology

Background:

  • Laser-based microdissection enhances cell procurement precision from tissue sections.
  • Traditional methods struggle with large sample requirements (e.g., proteomics) and identifying scattered cells.
  • Automated, immunostain-guided approaches are needed for complex biological samples.

Purpose of the Study:

  • To discuss the application of immunohistochemistry (IHC) and immunofluorescence (IF) in guiding laser microdissection.
  • To present methods for manual and automated immuno-based microdissection.
  • To highlight the utility of these techniques for challenging cell isolation scenarios.

Main Methods:

  • Utilizing immunohistochemistry (IHC) and immunofluorescence (IF) staining.
  • Employing manual microdissection guided by IHC/IF.
  • Implementing software-driven automated microdissection based on IHC/IF signals.
  • Adapting protocols for commercially available laser dissection systems.

Main Results:

  • Demonstrated successful immuno-based microdissection using laser systems.
  • Showcased the ability to isolate specific cell populations guided by antibody staining.
  • Addressed technical challenges and provided solutions for reproducible results.

Conclusions:

  • Immuno-based microdissection offers a powerful solution for isolating specific cells from complex tissues.
  • This automated approach is crucial for applications like proteomics and the study of rare cell types.
  • Further development and optimization of these methods will advance cell-based research.