Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of genomic regions and candidate genes for tuber micronutrient accumulation in potato (Solanum tuberosum L.) through GBS-based GWAS.

Scientific reports·2026
Same author

In silico-screened short amidated peptides engineered for dual bactericidal action and antibiotic delivery through self-assembly.

Bioorganic chemistry·2026
Same author

dsRADAR: Imaging and Quantifying Cellular dsRNA by Repurposing RNA Binding Proteins.

bioRxiv : the preprint server for biology·2026
Same author

ENDONUCLEASE V: FROM TRANSCRIPTOME REGULATOR TO CHEMICAL BIOLOGY TOOL.

ChemistryEurope·2026
Same author

Aeroponic root leachate (ARL)-induced hatching as a sustainable strategy for the management of Globodera rostochiensis in potato (Solanum tuberosum L.).

Scientific reports·2026
Same author

Development of Dual Aurora-A and Aurora-B Degrading PROTACs for <i>MCYN</i>-Amplified Neuroblastoma.

ChemMedChem·2025
Same journal

Radical Cascades on Seawater Microdroplets Drive Atmospheric Mercury Oxidation.

Journal of the American Chemical Society·2026
Same journal

Superior Selective and Fast NH<sub>3</sub> Adsorption of Soft Porous MOF/Ionic Liquid Composites with Ordering Phase Transitions.

Journal of the American Chemical Society·2026
Same journal

Systematic Catalyst Variation for Improved Stereoselective Epoxide Polymerization: Subtle Modifications Resulting in Superior Efficiency.

Journal of the American Chemical Society·2026
Same journal

Deciphering the Halide Chemistry of Cl<sup>-</sup> and Br<sup>-</sup> in Enhancing Kinetics of Mg Plating/Stripping.

Journal of the American Chemical Society·2026
Same journal

Electrosynthesis of C<sub>6</sub> Chemicals by Propylene Oxidative Coupling on Au Surface.

Journal of the American Chemical Society·2026
Same journal

Statistical AI Enables Precise Screening of Multielement Catalysts.

Journal of the American Chemical Society·2026
See all related articles

Related Experiment Video

Updated: May 31, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Small-molecule-dependent split aptamer ligation.

Ashwani K Sharma1, Jennifer M Heemstra

  • 1Department of Chemistry and the Center for Cell and Genome Science, University of Utah, Salt Lake City, Utah 84112, United States.

Journal of the American Chemical Society
|July 19, 2011
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel DNA ligation method controlled by small molecules. This DNA reaction, triggered by cocaine binding to a split aptamer, enables sensitive detection in biological fluids.

More Related Videos

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Related Experiment Videos

Last Updated: May 31, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Area of Science:

  • Chemical Biology
  • Molecular Biology
  • Biotechnology

Background:

  • Small-molecule binding events can be harnessed to control chemical processes.
  • DNA aptamers are versatile tools for molecular recognition.
  • Split aptamers offer a modular approach to aptamer design.

Purpose of the Study:

  • To demonstrate the first use of small-molecule binding to direct a chemical reaction between two nucleic acid strands.
  • To develop a novel DNA ligation assay for small-molecule detection.
  • To investigate the binding and assembly properties of a cocaine-specific split aptamer.

Main Methods:

  • Utilized strain-promoted azide-alkyne cycloaddition for DNA ligation.
  • Employed a DNA split aptamer designed for cocaine recognition.
  • Tested the assay's performance in human blood serum and under varying salt concentrations.

Main Results:

  • Achieved small-molecule-dependent DNA ligation triggered by cocaine.
  • Demonstrated dose-dependent ligation over a broad range of cocaine concentrations.
  • Gained new insights into the cocaine split aptamer's assembly and binding characteristics.

Conclusions:

  • Small-molecule-triggered DNA ligation is a viable strategy for developing novel detection assays.
  • The developed method is compatible with complex biological matrices like blood serum.
  • This approach holds promise for broadly applicable small-molecule sensing applications.