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Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Transposons01:24

Transposons

Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

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Updated: May 31, 2026

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

Advances in bacterial transcriptome and transposon insertion-site profiling using second-generation sequencing.

Melanie Febrer1, Kirsten McLay, Mario Caccamo

  • 1The Genome Analysis Centre, Norwich Research Park, Colney Lane, Norwich NR4 7UH, UK.

Trends in Biotechnology
|July 19, 2011
PubMed
Summary
This summary is machine-generated.

Second-generation sequencing dramatically advanced bacterial studies, enhancing understanding of bacterial adaptation, diversity, and disease. This review covers high-throughput tools like RNA sequencing and transposon monitoring for bacterial research.

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Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles
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Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri
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Last Updated: May 31, 2026

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Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles
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Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles

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Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri
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Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri

Published on: September 23, 2017

Area of Science:

  • Microbiology
  • Genomics
  • Bioinformatics

Background:

  • Second-generation sequencing technologies have rapidly transformed bacterial research.
  • These platforms offer high-throughput capabilities, replacing older methods like DNA cloning and microarrays.
  • Advancements enable deeper insights into bacterial development, adaptation, diversity, and pathogenesis.

Purpose of the Study:

  • To review the impact of second-generation sequencing on bacterial studies.
  • To outline key high-throughput tools and their in silico analyses.
  • To highlight current biological questions addressed by these approaches.

Main Methods:

  • RNA sequencing (RNA-Seq) for comprehensive transcriptome analysis.
  • Transposon monitoring for systematic gene function determination.
  • In silico analysis of large-scale sequencing data.

Main Results:

  • Revolutionized understanding of bacterial biology and disease mechanisms.
  • Enabled high-throughput analysis of bacterial transcriptomes and gene functions.
  • Facilitated more comprehensive and systematic bacterial research.

Conclusions:

  • Second-generation sequencing is a powerful tool in modern microbiology.
  • These technologies are crucial for addressing complex biological questions in bacteria.
  • The integration of sequencing and bioinformatics drives significant advancements in the field.