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Updated: Aug 14, 2026

Imaging Local Ca2+ Signals in Cultured Mammalian Cells
Published on: March 3, 2015
Inositol 1,4,5-trisphosphate activates two types of Ca2(+)-permeable channels in human carcinoma cells
G N Mozhayeva1, A P Naumov, Y A Kuryshev
1Institute of Cytology, Academy of Sciences of the USSR, Leningrad.
Abstract:
Ca2(+)-permeable channels in human carcinoma A431 cells were studied using the patch clamp technique. We have found two types of Ca2(+)-permeable channels which are activated by inositol 1,4,5-trisphosphate (IP3) applied to the intracellular side of the plasma membrane. Unitary conductances of these channels are 3.7 and 13 pS (105 mM Ca2+ in recording pipette, 30-33 degrees C). From the extracellular side of the membrane the channels are activated by EGF. It is assumed that extracellular agonists open both channel types by stimulating the release of IP3 from the membrane.
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