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Updated: May 30, 2026
08:53
Isolation and Transcriptome Analysis of Plant Cell Types
Published on: April 7, 2023
Transcriptome profiling and insilico analysis of Gynostemma pentaphyllum using a next generation sequencer
Sathiyamoorthy Subramaniyam1, Ramya Mathiyalagan, In Jun Gyo
1Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University, Yongin, 449-701, South Korea.
Plant Cell Reports
|July 20, 2011
Summary
Gynostemma pentaphyllum contains gynosaponins, similar to Panax ginseng
Area of Science:
- Plant biochemistry
- Genomics
- Phytochemistry
Background:
- Gynosaponins (Gypenosides) are key phytochemicals in Gynostemma pentaphyllum, structurally related to ginsenosides found in Panax ginseng.
- These terpenoid compounds constitute 25% of G. pentaphyllum's total gynosaponins, with significant similarity to ginsenosides.
Purpose of the Study:
- To identify secondary metabolite genes in G. pentaphyllum by analyzing its genome's transcriptional levels.
- To facilitate comparative transcriptomic analysis between G. pentaphyllum and P. ginseng.
Main Methods:
- Whole transcriptome sequencing of G. pentaphyllum roots and leaves using a GS-FLX pyro-sequencer.
- In silico analysis of obtained sequence reads for annotation and gene identification.
- Identification of secondary metabolite genes, particularly terpenoids.
Main Results:
- Acquisition and annotation of 265,340 transcriptome reads from G. pentaphyllum.
- Successful identification of numerous secondary metabolite genes, including mono-, di-, tri-, and sesquiterpenoids.
- Discovery of several terpenoid genes with similarities to those studied in P. ginseng.
Conclusions:
- The identified transcripts provide a foundation for characterizing more triterpenoid genes in G. pentaphyllum.
- This study enables a comparative transcriptomic understanding of G. pentaphyllum and P. ginseng.
- The findings contribute to understanding the biosynthesis of gynosaponins and their relationship to ginsenosides.
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