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Updated: May 30, 2026

Enzymatic Cascade Reactions for the Synthesis of Chiral Amino Alcohols from L-lysine
Published on: February 16, 2018
[Resolution of optical isomers of beta-amino ketone compounds with two chiral centres by high performance liquid
Pengbo Li1, Youpingi Tian, Liren Chen
1Key Laboratory of Macromolecular Science of Shaanxi Province, School of Chemistry & Materials Science, Shaanxi Normal University, Xi' an 710062, China.
Abstract:
A high performance liquid chromatographic method was developed for the resolution of optical isomers of beta-amino ketone compounds containing two chiral centres. An AE. Lichrom AM 1 chiral chromatographic column (250 mm x 4.6 mm, 5 microm) was used. The influences o the mobile phase polarity, flow rate and elution mode were investigated. The optimum conditions were the use of hexane-isopropyl alcohol as the mobile phase with a flow rate of 1 mL/min and gradient elution. Under the above chromatographic conditions, the optical isomers of four kinds beta-amino ketone compounds containing two chiral centres were baseline separated (Rs > 1.5). It was proved that the method is fast and easy to separate the optical isomers of beta-amino ketone compounds containing two chiral centres.
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