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A simple haemolytic method for quantitation of the delta endotoxin of Bacillus thuringiensis subsp. israelensis from
M K Majumdar1, D P Sikdar, A B Sarma
1Central Drug Laboratory, Calcutta, India.
The Journal of Applied Bacteriology
|August 1, 1990
Abstract:
A simple haemolytic assay method for quantitative estimation of the delta endotoxin of Bacillus thuringiensis subsp. israelensis from a crude preparation has been developed. The method has several advantages over mosquito-larvicidal methods of assay as it is inexpensive, highly sensitive and easier to run and can be used for performing a reasonably large number of assays rapidly with high precision and with a coefficient of variation that does not exceed 1.96%.