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Updated: May 30, 2026
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DNA Methylation: Bisulphite Modification and Analysis
Published on: October 21, 2011
On-chip DNA methylation analysis using osmium complexation
Kaori Sugizaki1, Tadashi Umemoto, Akimitsu Okamoto
1Nucleic Acid Chemistry Laboratory, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Journal of Nucleic Acids
|July 21, 2011
Abstract:
The development of a reaction for detecting the presence/absence of one methyl group in a long DNA strand is a chemically and biologically challenging research subject. A newly designed chemical assay on a chip for the typing of DNA methylation has been developed. A methylation-detection probe fixed at the bottom of microwells was crosslinked with methylated DNA mediated by osmium complexation and contributes to selective amplification of methylated DNA.
