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Updated: Aug 18, 2026

Specific Marking of HIV-1 Positive Cells using a Rev-dependent Lentiviral Vector Expressing the Green Fluorescent Protein
Published on: September 24, 2010
A tripartite HIV-1 tat-env-rev fusion protein
J Salfeld1, H G Göttlinger, R A Sia
1Dana-Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, MA 02115.
Abstract:
A 26 kd protein reactive with antiserum to the transactivator tat of the Human Immunodeficiency Virus Type 1 (HIV-1) has been detected in virus producing cells. The 26 kd protein is shown to be a tripartite fusion protein including coding sequences of the tat, envelope (env) and regulator of virion expression (rev) genes. Fusion of these coding sequences occurs by use of a previously undescribed exon within env. This 26 kd protein, designated tnv, has tat but no rev activity detectable with the assay used. The existence of other less abundant tat and rev related proteins in HIV-1 producing cells is also noted.
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