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Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...

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Related Experiment Video

Updated: May 30, 2026

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
06:21

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

Data quality in tissue analysis using desorption electrospray ionization.

Allison L Dill1, Livia S Eberlin, Anthony B Costa

  • 1Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.

Analytical and Bioanalytical Chemistry
|July 27, 2011
PubMed
Summary
This summary is machine-generated.

Sample storage conditions significantly impact mass spectrometry (MS) tissue imaging data quality. Storing tissue samples at room temperature or undergoing freeze/thaw cycles causes degradation, while -80°C storage is optimal for preserving lipid profiles.

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Last Updated: May 30, 2026

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
06:21

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
05:47

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Published on: February 19, 2020

Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry
12:49

Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry

Published on: April 4, 2018

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Molecular Imaging

Background:

  • Mass spectrometry (MS) is increasingly used for tissue analysis, especially lipid imaging.
  • Ambient ionization techniques like DESI-MS require careful sample handling for reliable data.
  • Understanding factors affecting data quality is crucial for reproducible results.

Purpose of the Study:

  • To investigate the impact of sample handling and storage on Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) data quality.
  • To evaluate the effects of pre-analytical variables on lipid profiles in mouse brain tissue.
  • To determine optimal conditions for preserving tissue integrity for MS analysis.

Main Methods:

  • Mouse brain tissue samples were subjected to various storage conditions (room temperature, -80°C, freeze/thaw cycles, heating).
  • Analytical variables including tissue thickness and drying time were tested.
  • Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) was used to analyze lipid profiles.

Main Results:

  • Room temperature storage for 24 hours led to degradation, evidenced by fatty acid dimer formation.
  • -80°C storage for 7 months showed minimal degradation compared to room temperature samples.
  • Multiple freeze/thaw cycles and heating (50°C) significantly increased sample degradation.
  • Tissue thickness (10-25 μm) and drying time (20 min) had minimal impact on data quality.
  • DESI-MS demonstrated high inter-section (0.44-7.2% RSD) and intra-sample (4.0-8.0% RSD) reproducibility.

Conclusions:

  • Sample storage conditions are the most critical factor affecting DESI-MS tissue data quality.
  • Room temperature storage leads to unacceptable sample degradation.
  • DESI-MS is a robust technique when appropriate sample handling and storage protocols are followed.