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Studies on polyoma virus DNA replication in synchronized C3H2K cells.
The Journal of General Virology
|February 1, 1979
Summary
Polyoma virus DNA replication in permissive cells is precisely timed during the S phase initiated by serum stimulation. Extensive host DNA replication is not required for efficient polyoma virus DNA replication.
Area of Science:
- Molecular Biology
- Virology
- Cell Cycle Regulation
Background:
- Polyoma virus infection requires host cell machinery for DNA replication.
- Cellular DNA synthesis is regulated by serum stimulation and cell cycle progression.
Purpose of the Study:
- To determine the precise timing of polyoma virus DNA replication competence during the cell cycle.
- To investigate the relationship between host DNA synthesis and viral DNA replication.
Main Methods:
- Infection of G1-arrested cells with polyoma virus at various times after serum stimulation.
- Monitoring of viral and cellular DNA synthesis.
- Assessing viral DNA replication in cells with inhibited host DNA synthesis.
Main Results:
- Polyoma virus DNA synthesis occurs in the first half of S phase in cells infected early after serum stimulation.
- Viral DNA replication is shifted to the subsequent S phase in cells infected later.
- Normal polyoma DNA yields are achieved even when host DNA synthesis is inhibited.
Conclusions:
- Permissive cells acquire competence for polyoma virus DNA replication at a specific point during serum-induced S phase.
- Serum stimulation can substitute for early viral functions in initiating DNA replication.
- Extensive host DNA replication is not an obligatory prerequisite for polyoma virus DNA replication.