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Cloning and expression of Thiobacillus versutus cryptic plasmid pTAV-1 DNA in Escherichia coli
E K Jagusztyn-Krynicka1, J Bielecki, B Kauc
1Institute of Microbiology, University of Warsaw, Poland.
Microbios
|January 1, 1990
Abstract:
pTAV-1 is an approximately 100 kb Thiobacillus versutus cryptic plasmid. pTAV-1 DNA was cloned in Escherichia coli. Nine recombinant plasmids containing pTAV-1 DNA inserted into the EcoRI restriction site of pACYC184 were constructed. The origin of DNA inserts was confirmed by Southern blot hybridization. The expression of mixotrophic T. versutus plasmid genes was demonstrated in E. coli.