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Updated: May 30, 2026
High-throughput Screening for Protein-based Inheritance in S. cerevisiae
Published on: August 8, 2017
[Screening and breeding high 1,3-propanediol producing strains by genome shuffling]
Shengguo Lu1, Shuang Li, Fang Luo
1Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650216, China.
Objective:
To improve the tolerance of main metabolites, we used genome shuffling to achieve high 1,3-propanediol producing mutants.
Methods:
Based on 96 deep-well palates containing prepared ended fed-batch broth as an efficient selection method, genome shuffling has been applied in strain improvement.
Results:
Five high producers were obtained after genome shuffling (LSG1, LSG2, LSG4, LSG5 and LSG6). During batch fermentation (3 L), the 1, 3-propanediol production of the five mutants were improved 17.0%, 19.0%, 12.9%, 23.9% and 18.0% , compared with the parent strain; the conservations from glycerol were improved 17.7%, 20.0%, 13.3%, 24.4% and 17.7%.
Conclusion:
Genome shuffling was an efficient approach for strain improvement, and 96 deep-well palates containing fed-batch broth has been demonstrated as an efficient selection approach.
