Effects of refluxate pH values on duodenogastroesophageal reflux-induced esophageal adenocarcinoma
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Updated: May 30, 2026

Surgical Models of Gastroesophageal Reflux with Mice
Published on: August 25, 2015
Peng Cheng1, Jian-Sheng Li, Jun Gong
1Department of Gastroenterology, First Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China. cpzxczzc2004@yahoo.com.cn
Aim:
To determine the effects of duodenogastric juice pH on the development of esophageal adenocarcinoma (EAC).
Methods:
An animal model of duodenogastroesophageal reflux was established using Sprague-Dawley (SD) rats undergoing esophagoduodenostomy (ED). The development of EAC was investigated in rats exposed to duodenogastric juice of different pH. The rats were divided into three groups: low-pH group (group A), high-pH group (group B) and a sham-operated group as a control (group C) (n = 30 rats in each group). The incidence of esophagitis, Barrett's esophagus (BE), intestinal metaplasia with dysplasia and EAC was observed 40 wk after the treatment.
Results:
The incidence rate of esophagitis, BE, intestinal metaplasia with dysplasia and EAC was higher in groups A and B compared with the control group after 40 wk (P < 0.01), being 96% and 100% (P > 0.05), 88% and 82.4% (P > 0.05), 20% and 52.1% (P < 0.05), and 8% and 39% (P < 0.05), respectively.
Conclusion:
Non-acidic refluxate increases the occurrence of intestinal metaplasia with dysplasia and EAC while the low-pH gastric juice exerts a protective effect in the presence of duodenal juice. The non-acid reflux is particularly important in the progression from BE to cancer. Therefore, control of duodenal reflux may be an important prophylaxis for EAC.
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