Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Innovative Approach to Rapid Growth of Highly Clay-Filled Coatings on Porous Polyurethane Foam.

ACS macro letters·2022
Same author

Efficient removal of formaldehyde using metal-biochar derived from acid mine drainage sludge and spent coffee waste.

Journal of environmental management·2021
Same author

Bacterial target-specific photocatalyst for the enhancement of antibacterial property to targets.

Applied catalysis. B, Environmental·2020
Same author

The simultaneous capture of mercury and fine particles by hybrid filter with powder activated carbon injection.

Environmental pollution (Barking, Essex : 1987)·2018
Same author

Smoldering and Flame Resistant Textiles <i>via</i> Conformal Barrier Formation.

Advanced materials interfaces·2017
Same author

Pilot-scale evaluation of a novel TiO<sub>2</sub>-supported V<sub>2</sub>O<sub>5</sub> catalyst for DeNOx at low temperatures at a waste incinerator.

Waste management (New York, N.Y.)·2016
Same journal

A two-step centrifugal microfluidic platform for semi-automated IGRA detection of tuberculosis based on chemiluminescence.

The Analyst·2026
Same journal

On-site rapid identification of animal and plant creams <i>via</i> 2D FeB nanozyme-based colorimetric sensors.

The Analyst·2026
Same journal

Sensitive detection of aflatoxin B1 using a dual-mode fluorescent aptasensor based on cascade signal amplification.

The Analyst·2026
Same journal

Deep learning-enabled microfluidic digital PCR platform for efficient seven-color quantification.

The Analyst·2026
Same journal

Monitoring food spoilage biogenic amines utilizing a blue-emitting fluorescent ionic liquid.

The Analyst·2026
Same journal

Correction: Regeneration-on-a-chip: a planarian microfluidic device enabling automated cultivation, individual tracking and <i>in vivo</i> imaging for regeneration study.

The Analyst·2026
See all related articles

Related Experiment Video

Updated: May 30, 2026

Design and Development of Aptamer&#8211;Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
08:23

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

Gold nanoparticle-based homogeneous fluorescent aptasensor for multiplex detection.

Yeon Seok Kim1, Jongsoo Jurng

  • 1Environment Division, Korea Institute of Science and Technology, 39-1 Hawolgok-dong, Seongbuk-gu, Seoul, 136-791, South Korea.

The Analyst
|July 30, 2011
PubMed
Summary
This summary is machine-generated.

This study presents a novel fluorescence assay for detecting multiple analytes simultaneously. The assay utilizes DNA aptamers that change shape to signal the presence of targets like mercury ions, adenosine, and potassium ions.

More Related Videos

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

Related Experiment Videos

Last Updated: May 30, 2026

Design and Development of Aptamer&#8211;Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
08:23

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Multiplex detection assays are crucial for simultaneous analysis of multiple analytes.
  • Quantum dots (QDs) and gold nanoparticles (AuNPs) offer unique optical properties for biosensing.
  • DNA aptamers provide high specificity and stability for molecular recognition.

Purpose of the Study:

  • To develop a homogeneous fluorescence assay for multiplex detection.
  • To utilize target-induced conformational changes of DNA aptamers for signal generation.
  • To achieve simultaneous detection of mercury ions, adenosine, and potassium ions.

Main Methods:

  • Immobilizing DNA aptamers on quantum dots (QDs).
  • Adsorbing QD-aptamer conjugates onto gold nanoparticles (AuNPs) via electrostatic interactions.
  • Utilizing Förster Resonance Energy Transfer (FRET) for fluorescence quenching and recovery.
  • Employing target-induced aptamer conformational changes to detach conjugates from AuNPs.

Main Results:

  • A homogeneous fluorescence assay was successfully developed.
  • The assay demonstrated effective fluorescence quenching by AuNPs through FRET.
  • Target binding induced aptamer conformational changes, leading to fluorescence recovery proportional to concentration.
  • Simultaneous detection of mercury ions, adenosine, and potassium ions was achieved with high selectivity.

Conclusions:

  • The developed assay enables sensitive and selective multiplex detection of various analytes.
  • The QD/aptamer-AuNP system offers a robust platform for homogeneous biosensing.
  • This approach holds promise for applications in environmental monitoring and clinical diagnostics.