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Updated: May 30, 2026

Plasma Lithography Surface Patterning for Creation of Cell Networks
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Epithelial cell patterns on a PDMS polymer surface using a micro plasma structure.

Jung-Ho Kim1, Soonmin Seo, Junhong Min

  • 1Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentisty, Kyung Hee University, Seoul 130-701, Republic of Korea.

Journal of Biotechnology
|August 2, 2011
PubMed
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Researchers created a novel epithelial cell-patterning tool using micro plasma on polydimethylsiloxane (PDMS) surfaces. This method enables precise cell adhesion without chemical treatments, simplifying tissue engineering applications.

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Microfluidics

Background:

  • Cell adhesion and patterning are crucial for tissue engineering and biological studies.
  • Conventional methods often involve complex chemical or biological treatments.
  • Polydimethylsiloxane (PDMS) is a widely used polymer in microfluidics and tissue engineering.

Purpose of the Study:

  • To develop a simple and reproducible method for epithelial cell patterning on PDMS surfaces.
  • To investigate the use of micro plasma technology for cell adhesion control.
  • To achieve precise single-cell and closed-cell pattern formation.

Main Methods:

  • Fabrication of a micro plasma structure using copper and SU-8 photoresist on a glass substrate.
  • Utilizing a micro plasma chamber to create a distinct space between a copper electrode and the PDMS surface.

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Last Updated: May 30, 2026

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  • Employing a micro-plasma structure of 30 μm width for single cell patterning.
  • Developing a 200 μm diameter micro chamber with a 10 μm microchannel for closed cell patterns.

    • Main Results:

    • Demonstrated successful epithelial cell patterning on PDMS surfaces without chemical or biological treatment.
    • Showcased preferential cell adhesion to plasma-treated PDMS areas compared to untreated areas.
    • Achieved precise single-cell patterning with a 30 μm micro plasma structure.
    • Successfully created closed cell patterns within a 200 μm micro chamber with a 10 μm microchannel.

    Conclusions:

    • The developed micro plasma tool offers a simple, reproducible, and effective method for epithelial cell patterning on PDMS.
    • This technique eliminates the need for chemical or biological treatments, streamlining cell culture and tissue engineering workflows.
    • The ability to create precise single-cell and closed-cell patterns opens new possibilities for studying cell-cell interactions and developing complex tissue constructs.