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Updated: May 30, 2026

A Study of the Complexation of Mercury(II) with Dicysteinyl Tetrapeptides by Electrospray Ionization Mass Spectrometry
Published on: January 8, 2016
Serum/plasma methylmercury determination by isotope dilution gas chromatography-inductively coupled plasma mass
Douglas C Baxter1, Mikko Faarinen, Heléne Österlund
1ALS Scandinavia AB, Aurorum 10, 977 75 Luleå, Sweden. douglas.baxter@alsglobal.com
Abstract:
A method for the determination of methylmercury in plasma and serum samples was developed. The method uses isotope dilution with (198)Hg-labeled methylmercury, extraction into dichloromethane, back-extraction into water, aqueous-phase ethylation, purge and trap collection, thermal desorption, separation by gas chromatography, and mercury isotope specific detection by inductively coupled plasma mass spectrometry. By spiking 2 mL sample with 1.2 ng tracer, measurements in a concentration interval of (0.007-2.9) μg L(-1) could be performed with uncertainty amplification factors <2. A limit of quantification of 0.03 μg L(-1) was estimated at 10 times the standard deviation of concentrations measured in preparation blanks. Within- and between-run relative standard deviations were <10% at added concentration levels of 0.14 μg L(-1), 0.35 μg L(-1) and 2.8 μg L(-1), with recoveries in the range 82-110%. Application of the method to 50 plasma/serum samples yielded a median (mean; range) concentration of methylmercury of 0.081 (0.091; <0.03-0.19) μg L(-1). This is the first time methylmercury has been directly measured in this kind of specimen, and is therefore the first estimate of a reference range.
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