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Sensitive sandwich electrochemical immunosensor for alpha fetoprotein based on prussian blue modified hydroxyapatite
Yuxue Dai1, Yanyan Cai, Yanfang Zhao
1School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, People's Republic of China.
Biosensors & Bioelectronics
|August 2, 2011
Summary
A novel sandwich electrochemical immunosensor utilizing Prussian blue modified hydroxyapatite (PB@HAP) and horse radish peroxidase (HRP) offers sensitive alpha fetoprotein (AFP) detection. This biosensor demonstrates high sensitivity and stability for tumor marker analysis.
Area of Science:
- Biomedical Engineering
- Biosensors
- Electrochemistry
Background:
- Alpha fetoprotein (AFP) is a crucial tumor marker for liver cancer and other conditions.
- Sensitive and selective detection methods are vital for early diagnosis and monitoring.
- Existing immunosensors face challenges in sensitivity, stability, and cost-effectiveness.
Purpose of the Study:
- To develop a highly sensitive sandwich electrochemical immunosensor for alpha fetoprotein (AFP) detection.
- To utilize Prussian blue modified hydroxyapatite (PB@HAP) as an electrochemical label for enhanced signal amplification.
- To investigate the potential of this immunosensor for clinical applications and mass production.
Main Methods:
- Fabrication of a sandwich electrochemical immunosensor using PB@HAP-HRP-Ab(2) as the label and a graphene sheet (GS)/thionine (TH) mixture for antibody immobilization.
- Electrochemical detection of AFP using amperometric measurements.
- Characterization of the immunosensor's performance, including sensitivity, selectivity, and stability.
- Validation using human serum samples.
Main Results:
- The developed immunosensor achieved a high sensitivity for AFP detection, with a linear range of 0.02-8 ng/mL and a low detection limit of 9 pg/mL.
- The PB@HAP-HRP-Ab(2) label demonstrated significantly higher sensitivity compared to other tested labels.
- The immunosensor exhibited excellent selectivity, stability, and satisfactory performance in analyzing AFP in serum samples.
- The use of screen-printed electrodes with PB@HAP offers potential for cost-effective mass production.
Conclusions:
- The fabricated sandwich electrochemical immunosensor provides a sensitive, selective, and stable platform for AFP determination.
- The novel PB@HAP-based electrochemical label significantly enhances detection performance.
- This technology holds promise for early cancer diagnosis and has potential applications for detecting other tumor markers.
