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Effects of insulator cHS4 on transgene expression from plasmid DNA in a positive feedback system
Hiroshi Ochiai1, Hideyoshi Harashima, Hiroyuki Kamiya
1Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.
Journal of Bioscience and Bioengineering
|August 2, 2011
Abstract:
The effects of a genetic insulator (chicken cHS4) on transgene expression in a positive feedback system using the GAL4-VP16 activator and luciferase reporter plasmids were examined. The introduction of cHS4 enhanced luciferase expression in mouse liver, indicating that this system would be useful for efficient transgene expression.
