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Updated: May 30, 2026

Forward Genetic Approaches in Chlamydia trachomatis
Published on: October 23, 2013
Molecular diagnosis of genital Chlamydia trachomatis infection by polymerase chain reaction
E R Khan1, M A Hossain, S K Paul
1Department of Microbiology, Mymensingh Medical College, Mymensingh, Bangladesh.
Abstract:
The present study was carried to diagnose genital Chlamydia trachomatis infection among women of reproductive age, attending Mymensingh Medical College Hospital, during July 2009 to June 2010 by Polymerase chain reaction (PCR). A total of 70 females were investigated including 56 symptomatic and 14 asymptomatic cases. Endocervical swabs were collected and dipped in 500μl Tris buffer. Polymerase chain reaction (PCR) amplification was done for detection of endogenous plasmid-based nucleic acid. A total 17 out of 56 of the symptomatic cases (30.4%) were positive for C. trachomatis and none were found positive among the 14 asymptomatic cases. These findings suggest high prevalence of C. trachomatis infection among this group of population.
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