Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Label Type Influence on DNA Translocation Velocity in Solid-State Nanopores.

ACS nano·2026
Same author

Membrane-Spanning Nanopores Formed from Nucleic Acids.

Chemical reviews·2026
Same author

Modular RNA:DNA Nanostructures Enable Nanopore Profiling of rRNA Processing and rRNA Variants.

ACS nano·2026
Same author

Reconfigurable Multichannel Glass Nanopores Speed up DNA and MicroRNA Detection.

Nano letters·2026
Same author

Interaction of Pf4 tactoids with bacteria and synthetic colloidal rods.

European biophysics journal : EBJ·2026
Same author

Quantification of disease-associated RNA tandem repeats by nanopore sensing.

Nature communications·2026

Related Experiment Video

Updated: May 30, 2026

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
15:06

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

Parallel sub-micrometre channels with different dimensions for laser scattering detection.

Stefano Pagliara1, Catalin Chimerel, Richard Langford

  • 1University of Cambridge, Cavendish Laboratory, Cambridge, CB3 0HE, United Kingdom.

Lab on a Chip
|August 2, 2011
PubMed
Summary

Researchers developed a simple method for creating polymer microchannels using focused ion beam-deposited platinum wires. This technique enables precise control over channel dimensions for particle analysis and sensing applications.

More Related Videos

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels
11:34

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Published on: September 8, 2016

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Related Experiment Videos

Last Updated: May 30, 2026

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
15:06

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels
11:34

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Published on: September 8, 2016

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Area of Science:

  • Materials Science and Engineering
  • Nanotechnology
  • Microfluidics

Background:

  • Fabrication of sub-micrometre channels is crucial for advanced microfluidic applications.
  • Existing methods for creating such small channels can be complex and costly.
  • Need for precise control over channel dimensions for particle manipulation and analysis.

Purpose of the Study:

  • To introduce a novel and simple approach for fabricating polymer sub-micrometre channels.
  • To characterize the transport of polymer colloids within these fabricated channels.
  • To demonstrate a prototype application for in situ particle sizing and sensing.

Main Methods:

  • Utilized replica molding of platinum (Pt) wires deposited by focused ion beam (FIB) for channel fabrication.
  • Fabricated arrays of parallel polymer channels with dimensions down to 600 nm and variable height.
  • Employed laser scattering detection to characterize pressure-driven transport of polymer colloids.

Main Results:

  • Successfully fabricated polymer channels with sub-micrometre dimensions and controlled height.
  • Characterized polymer colloid translocation frequency, amplitude, and duration within the channels.
  • Demonstrated the capability for in situ sizing and sensing of particles down to 50 nm.

Conclusions:

  • The FIB-replica molding technique provides a simple and effective route to polymer sub-micrometre channels.
  • The developed platform allows for detailed characterization of particle transport at the microscale.
  • This technology holds promise for applications in particle sizing, sensing, and microfluidic analysis.