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Published on: June 26, 2018
Detection of gene expression in mouse embryos and tissue sections
Edwina McGlinn1, Jennifer H Mansfield
1Department of Genetics, Harvard Medical School, Boston, MA, USA. emcglinn@receptor.med.harvard.edu
Methods in Molecular Biology (Clifton, N.J.)
|August 2, 2011
Summary
This chapter details methods for detecting gene expression in mouse embryonic development. It covers techniques like mRNA in situ hybridization and immunohistochemistry for analyzing gene activity.
Area of Science:
- Developmental Biology
- Molecular Biology
- Genetics
Background:
- Gene expression analysis is crucial for understanding embryonic development.
- Studying gene patterns in mouse embryos provides insights into developmental processes.
Purpose of the Study:
- To detail methods for detecting gene expression in whole mouse embryos and tissue sections.
- To describe commonly used techniques for analyzing gene activity in mouse models.
Main Methods:
- mRNA in situ hybridization for detecting specific RNA transcripts.
- Immunohistochemistry for visualizing protein localization and expression.
- Detection of enzymatic and fluorescent protein reporters for gene activity monitoring.
Main Results:
- The chapter provides a comprehensive overview of established gene expression analysis techniques.
- It highlights the utility of these methods in studying mouse embryonic development.
Conclusions:
- Accurate gene expression analysis is fundamental to advancing our understanding of embryonic development.
- The described methods offer powerful tools for researchers investigating developmental processes in mice.
