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Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

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Related Experiment Video

Updated: May 30, 2026

Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Gene targeting in the mouse.

Anne E Griep1, Manorama C John, Sakae Ikeda

  • 1Department of Cell and Regenerative Biology, University of Wisconsin-Madison, Madison, WI, USA. aegriep@wisc.edu

Methods in Molecular Biology (Clifton, N.J.)
|August 2, 2011
PubMed
Summary
This summary is machine-generated.

Gene-targeting in embryonic stem cells enables the creation of knock-out mice for studying gene function. Advanced methods now allow for conditional knock-out and knock-in mouse generation.

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Related Experiment Videos

Last Updated: May 30, 2026

Mouse Genome Engineering Using Designer Nucleases
12:04

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Published on: April 2, 2014

Generation of Genetically Modified Mice through the Microinjection of Oocytes
10:19

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models
10:57

CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models

Published on: August 24, 2022

Area of Science:

  • Genetics
  • Molecular Biology

Background:

  • Homologous recombination in embryonic stem (ES) cells is a powerful tool for genetic manipulation.
  • The generation of knock-out mice has significantly advanced the study of gene function in vivo.

Purpose of the Study:

  • To provide an overview of gene-targeting strategies.
  • To discuss procedures for generating genetically engineered mice.

Main Methods:

  • Gene inactivation via homologous recombination in ES cells.
  • Development of advanced strategies for conditional knock-out and knock-in mice.

Main Results:

  • Successful generation of numerous null mutant (knock-out) mice.
  • Refinement of gene-targeting technology for more precise genetic engineering.

Conclusions:

  • Gene-targeting is a versatile tool for understanding gene function across diverse biological research areas.
  • Advanced techniques like conditional knock-out and knock-in offer enhanced capabilities for genetic studies.