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Updated: May 30, 2026
Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium
Published on: May 18, 2021
[Prokaryotic expression and polyclonal antibody preparation of human Nodal mature peptide]
Ling-ling Li1, Guan-min Jiang, Ge Zhang
1School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.
Aim:
To express the fusion protein of human Nodal mature peptide in prokaryocytes and to prepare mouse anti-Nodal polyclonal antibody.
Methods:
The expression vector pReceiver-hNodal was constructed and transformed into BL21(DE3). His(6);-hNodal fusion protein was induced by IPTG and separated on SDS-PAGE and the recovered fusion protein was used to immune BALB/c to produce polyclonal antibody. The titer of the polyclonal antibody was detected by indirect ELISA and its specificity was analyzed byWestern blot and immunochemical staining.
Results:
The expected molecular weight of fusion protein around 15 000 was highly expressed in E.coli and specific antibody was obtained.
Conclusion:
The successful preparation of anti-Nodal mature peptide polyclonal antibody will lay the basis for further study of the biological function of Nodal on tumor progress.
