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In situ hybridization to polytene chromosomes in Drosophila using digoxigenin-labeled probes

Mary-Lou Pardue

    Cold Spring Harbor Protocols
    |August 3, 2011
    PubMed
    Abstract

    No abstract available in PubMed .

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    In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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