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Related Experiment Video

Updated: May 30, 2026

Quantifiable and Inexpensive Cell-Free Fluorescent Method to Confirm the Ability of Novel Compounds to Chelate Iron

Quantifiable and Inexpensive Cell-Free Fluorescent Method to Confirm the Ability of Novel Compounds to Chelate Iron

Published on: February 23, 2024

A turn-on fluorescent iron complex and its cellular uptake.

Jy D Chartres¹, Michael Busby, Mark J Riley

¹School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland, Australia 4072.

Inorganic Chemistry

|August 5, 2011

Summary

Researchers developed a fluorescent iron chelator to track iron within cells. This new ligand, L(1), helps visualize how iron moves in cells, aiding the study of iron overload disorders.

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Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

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Quantifiable and Inexpensive Cell-Free Fluorescent Method to Confirm the Ability of Novel Compounds to Chelate Iron

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Published on: February 23, 2024

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Published on: September 8, 2009

Area of Science:

Biochemistry
Cell Biology
Medicinal Chemistry

Background:

Chronic iron overload disorders necessitate chelators that can enter cells, bind labile iron, and be excreted.
The intracellular labile iron pools and their mechanisms remain poorly understood.
Heterocyclic hydrazine-based chelators like benzoyl picolinoyl hydrazine (H(2)BPH) show promise in mobilizing intracellular iron.

Purpose of the Study:

To develop a fluorescent probe for visualizing intracellular iron complexation and transport.
To characterize a novel fluorescent chelator and its iron(III) complex.
To investigate the cellular uptake and localization of the iron-chelator complex.

Main Methods:

Synthesis and characterization of a novel fluorescent chelator (L(1)) by grafting rhodamine B onto H(2)BPH.
Spectroscopic and X-ray crystallographic analysis of the free ligand and its 1:2 iron(III) complex, [Fe(III)(L(1))(2)](3+).
Confocal fluorescence microscopy to observe cellular uptake and localization in HeLa cells.

Main Results:

A novel fluorescent chelator, L(1), was synthesized, which is nonfluorescent alone but strongly fluorescent upon complexation with Fe(III).
The structure of the free ligand and the 1:2 iron(III) complex, [Fe(III)(L(1))(2)](3+), were confirmed.
Confocal microscopy demonstrated rapid cellular entry of the [Fe(III)(L(1))(2)](3+) complex into HeLa cells, with localization in endosomes/lysosomes.

Conclusions:

The developed fluorescent chelator L(1) enables visualization of iron complexation and cellular trafficking.
The study provides insights into the cellular behavior of iron chelators, relevant for treating iron overload.
The findings support the potential of such fluorescent probes in studying intracellular iron dynamics.