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Functional interactions between polydnavirus and host cellular innexins.

N K Marziano¹, D K Hasegawa, P Phelan

¹School of Biosciences, University of Kent, Canterbury, Kent, UK.

Journal of Virology

|August 5, 2011

Summary

This summary is machine-generated.

Polydnaviruses, like Campoletis sonorensis ichnovirus (CsIV), alter caterpillar physiology. CsIV vinnexins form functional gap junctions, potentially causing cellular pathologies by altering intercellular communication.

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Area of Science:

Virology
Molecular Biology
Insect Pathology

Background:

Polydnaviruses are double-stranded DNA viruses transmitted by parasitoid wasps.
These viruses infect host caterpillars, causing physiological disruptions through gene expression.
The Campoletis sonorensis ichnovirus (CsIV) encodes four innexin homologues, termed vinnexins.

Purpose of the Study:

To investigate the functionality of CsIV vinnexinQ1 and vinnexinQ2 in forming gap junctions.
To examine the interactions between CsIV vinnexins and the host caterpillar's Innexin2.
To determine if vinnexin-Innexin2 interactions alter gap junctional communication.

Main Methods:

Expression of vinnexinQ1 and vinnexinQ2 in paired Xenopus oocytes to assess junction formation.
Co-expression of vinnexins with Spodoptera frugiperda Innexin2 in cell pairs.
Functional analysis of gap junctions formed by vinnexins alone, Innexin2 alone, or in combination.

Main Results:

VinnexinQ1 and vinnexinQ2 form functional gap junctions in a heterologous system.
Vinnexins interact differentially with the insect Innexin2 orthologue.
Co-expression of vinnexins and Innexin2 results in functional junctions with distinct properties compared to Innexin2 alone.

Conclusions:

CsIV vinnexins are functional gap junction proteins.
Altered gap junctional intercellular communication due to vinnexin-Innexin2 interactions may contribute to ichnovirus-induced cellular pathologies in caterpillars.