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Updated: May 30, 2026
09:24
3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer
Published on: September 13, 2018
Epidermal growth factor receptor expression in urinary bladder cancer
Dayalu S L Naik1, Shashi Sharma, Amitabha Ray
1Institute of Cytology and Preventive Oncology, I-7, Sector-39, Noida, India.
Summary
This study investigated epidermal growth factor receptor (EGFR) expression in bladder cancer, finding no overall link with other markers. However, a significant association between EGFR and human epidermal growth factor receptor 2 (HER2) was noted in male patients.
Area of Science:
- Oncology
- Molecular Biology
- Urology
Background:
- Epidermal growth factor receptor (EGFR) and human epidermal growth factor receptor 2 (HER2) are key growth factor receptors implicated in various cancers.
- Understanding their expression patterns and interactions in urinary bladder cancer is crucial for targeted therapy development.
Purpose of the Study:
- To evaluate the expression of EGFR in urinary bladder cancer.
- To assess the association of EGFR with HER2, epidermal growth factor (EGF), interleukin-6 (IL-6), and high-risk human papilloma virus (HPV) types 16 and 18.
- To explore potential therapeutic targets in bladder cancer.
Main Methods:
- Immunohistochemical staining was used to evaluate EGFR, HER2, EGF, and IL-6 expression in 30 urothelial carcinoma cases.
- Polymerase chain reaction (PCR) was employed for HPV DNA detection.
- Western blot analysis was performed to assess intracellular molecules in EGFR signaling pathways.
Main Results:
- Immunopositivity rates were: EGFR (23%), HER2 (60%), EGF (47%), and IL-6 (80%).
- HPV-16 was detected in 10% of cases; HPV-18 was not detected.
- No significant overall association was found between EGFR expression and the studied parameters, except for a significant association with HER2 in male patients.
Conclusions:
- Overexpression of EGFR and/or HER2 was observed in a substantial portion of urinary bladder cancer cases.
- These findings highlight the importance of EGFR and HER2 in bladder cancer.
- Further understanding of these receptor interactions may aid in developing effective treatment strategies.
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