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Updated: May 30, 2026

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
Published on: February 7, 2019
Transcription factor beacons for the quantitative detection of DNA binding activity
Alexis Vallée-Bélisle1, Andrew J Bonham, Norbert O Reich
1Department of Chemistry and Biochemistry, University of California, Santa Barbara, 93106, United States.
Abstract:
The development of convenient, real-time probes for monitoring protein function in biological samples represents an important challenge of the postgenomic era. In response, we introduce here "transcription factor beacons," binding-activated fluorescent DNA probes that signal the presence of specific DNA-binding activities. As a proof of principle, we present beacons for the rapid, sensitive detection of three transcription factors (TATA Binding Protein, Myc-Max, and NF-κB), and measure binding activity directly in crude nuclear extracts.
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