Direct fabrication of nanoscale bio-adhesive patterns by electron beam surface modification of plasma polymerized...

In this study we present a method to produce nanostructured surfaces containing bio-adhesive features inside a non bio-adhesive matrix. The strategy is based on the combination of low pressure plasma polymerization and electron beam lithography processes and allows the fabrication of the structured materials in just two steps without using any solvents. In a first step, a thin protein-and-cell-repelling coating (∼10 nm) is obtained by plasma polymerization of Di-glyme. Then, in a second step, the bio-adhesive properties of the layer are tuned by monitoring the concentration of ether bonds of the film by irradiating it locally by different irradiation doses with an electron beam. Time-of-flight secondary ion mass spectroscopy and atomic force microscopy analysis have been used to characterize the produced surfaces. Experiments with a model protein (bovine serum albumin) on the patterned surfaces show preferential adhesion to the irradiated regions, indicating the potential of this simple technique for the development of highly compacted sensitive bio-sensing devices.