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Related Concept Videos

Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer is an enzyme that can...
Chromatin Packaging02:21

Chromatin Packaging

Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter? 
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
Chromatin Packaging01:32

Chromatin Packaging

Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
Chromatin Packaging02:21

Chromatin Packaging

Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter? 
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

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Related Experiment Video

Updated: May 30, 2026

Chromatin Interaction Analysis with Paired-End Tag Sequencing (ChIA-PET) for Mapping Chromatin Interactions and Understanding Transcription Regulation
21:55

Chromatin Interaction Analysis with Paired-End Tag Sequencing (ChIA-PET) for Mapping Chromatin Interactions and Understanding Transcription Regulation

Published on: April 30, 2012

Where splicing joins chromatin.

Jarmila Hnilicová1, David Staněk

  • 1Department of RNA Biology, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague.

Nucleus (Austin, Tex.)
|August 6, 2011
PubMed
Summary
This summary is machine-generated.

Gene expression involves transcription and splicing, which closely influence each other. Recent findings show chromatin and histone modifications significantly impact pre-messenger RNA (mRNA) splicing, affecting transcription rates and recruiting splicing factors.

Keywords:
alternative splicingchromatinexonhistone acetylationhistone methylationnucleosomesnRNPtranscription

More Related Videos

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
22:27

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Published on: May 6, 2010

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Last Updated: May 30, 2026

Chromatin Interaction Analysis with Paired-End Tag Sequencing (ChIA-PET) for Mapping Chromatin Interactions and Understanding Transcription Regulation
21:55

Chromatin Interaction Analysis with Paired-End Tag Sequencing (ChIA-PET) for Mapping Chromatin Interactions and Understanding Transcription Regulation

Published on: April 30, 2012

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
22:27

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Published on: May 6, 2010

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Gene Regulation

Background:

  • Transcription and pre-messenger RNA (mRNA) splicing are key gene expression steps.
  • Chromatin modifications were known to regulate transcription.
  • Emerging evidence highlights the role of chromatin and histone modifications in pre-mRNA splicing.

Purpose of the Study:

  • To summarize the interactions between the splicing machinery and chromatin.
  • To discuss the potential functional significance of these interactions.
  • To focus on histone acetylation and methylation in splicing.

Main Methods:

  • Literature review and synthesis of existing data.
  • Analysis of studies on chromatin modifications and splicing.
  • Focus on histone acetylation and methylation mechanisms.

Main Results:

  • Histone acetylation primarily influences splicing by altering transcription rates.
  • Histone methylation can directly impact splicing by recruiting splicing components.
  • Interactions between splicing machinery and chromatin are crucial for gene expression regulation.

Conclusions:

  • Chromatin modifications, particularly histone acetylation and methylation, play a significant role in pre-mRNA splicing.
  • Histone methylation offers a direct mechanism for influencing splicing beyond transcription rate modulation.
  • Understanding these interactions provides deeper insights into gene expression regulation.