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An evaluation of a solid phase red cell adherence test for detecting platelet-associated IgG in immune
1Department of Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, California 90048.
Abstract:
A solid phase red cell adherence (SPRCA) test, designed for platelet cross-match testing, was evaluated to determine its efficacy in detecting platelet-associated IgG (PAIgG) in immune thrombocytopenic purpura (ITP). This new method was compared to the platelet suspension immunofluorescence (PSIF) test. Fifty-three patient samples were tested by both methods, including 17 for whom a clinical diagnosis of ITP had been made. The SPRCA method showed 65% sensitivity, whereas the PSIF method showed 53% sensitivity. The specificity for both was 100%. The SPRCA compared favorably to the PSIF test in sensitivity and cost effectiveness. The SPRCA test is rapid, easy to perform, and suitable for detecting PAIgG in ITP.