[Study on yohimbine in positive mode by ion trap mass spectrometry]

The structure and fragmentation pathway of yohimbine were elucidated by electron spray ionization mass spectrometry( ESI-MS).

Quasi-molecular ion peak m/z 355 [M + H]+ was detected by ESI-MS, and the main fragment ions of m/z 212 and m/z 144 were detected by ESI-MS2.

There are two main fragment pathway for m/z 355 [M + H]+ by ESI-MS2 and the fragment broken in pyridine ring. The full scan MS3 spectra of fragment m/z 212, and m/z 144 was obtained by ion trap mass spectrometry. The characteristic fragmentation was used to prove the structure of m/z 212, and m/z 144. The fragment routes of characteristic were discussed on the basis of ESI mass spectra.

It can provide the experimental data for studying pharmacokinetics in vivo and modifying structure.